Description
Key facts
Detection method:Fluorescent,
Sample types:Adherent cells, Suspension cells,
Assay type:Cell-based (quantitative),
Assay Platform:Microplate reader, Fluor. microscope, Flow cyt.
Product details:
2-NBDG Glucose Uptake Assay Kit ab235976 provides a convenient tool for studying cellular glucose uptake. The kit uses 2-NBDG, a fluorescently-labeled deoxyglucose analog, as a probe for the detection of glucose taken up by cultured cells.
The 2-NBDG assay kit is suitable for analysis with flow cytometer, fluorescent microscope or microplate reader.
2-NBDG protocol summary:
- treat cells with experimental treatment in glucose-free medium
- add 2-NBDG and incubate for 10 min
- remove cell culture supernatant and wash cells with cell-based assay buffer
- add propidium iodide at this point to exclude dead cells if analyzing with flow cytometry
- add cell-based assay buffer and analyze immediately
2-NBDG can be detected with filters designed to detect FITC (Ex/Em 485/535 nm).
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-Multi, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
