Description
Key facts
Detection method:Colorimetric,
Sample types:Plasma, Serum,
Reacts with:Human,
Assay type:Quantitative,
Sensitivity:= 10 ng/mL,
Range:30 - 1000 ng/mL,
Assay Platform:Microplate
Product details:
Adalimumab ELISA Kit ab237641 is an indirect ELISA to measure Adalimumab in serum, plasma with a sensitivity of 10 ng/mL.
How the assay works:
Note: Adalimumab ELISA kit ab237641 is intended for Research Use Only, not for use in diagnostic procedures.
This kit is an indirect based ELISA kit. The color developed is proportional to the amount of Adalimumab in the sample or standard. Results of samples can be determined directly using the standard curve.
Assay Specificity
Our ELISA kits are rigorously validated to ensure the highest level of consistency and reproducibility. Please check the protocol booklet for more details
Adalimumab ELISA Kit ab237641 protocol summary
1. Add 100 µL Assay buffer to each well and add 20 µL standard or sample to appropriate wells. Cover and incubate for 30 minutes at room temperature
2. Discard incubation solution and wash plate 3 times with 300 µL diluted Wash Buffer
3. Add 100 µL HRP-conjugate to each well. Cover and incubate for 30 minutes at room temperature
4. Discard the solution and wash plate 3 times with 300 µL diluted Wash Buffer
5. Add 100 µL TMB Substrate and incubate the plate in the dark at room temperature for 10 minutes
6. Add 100 µL Stop Solution and read OD at 450 nm within 20 minutes.
This product is manufactured by BioVision, an Abcam company and was previously called E4372 BioSim™ Adalimumab (Human) ELISA Kit. E4372-100 is the same size as the 96 test size of ab237641.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
