Description
Key facts
Detection method:Colorimetric,
Assay type:Sandwich (quantitative),
Sensitivity:= 30 ng/mL,
Range:3 - 810 ng/mL,
Assay time:3h,
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
E. coli HCP ELISA Kit (host cell protein) ab240997 is a sandwich ELISA used to measure impurites caused by E. coli host cell proteins (HCP) with a sensitivity of 30 ng/mL.
How the assay works
The provided 96-well plate has been pre-coated with Rabbit anti-E. coli HCP antibodies to capture E. coli proteins from biological samples. Following an incubation to allow capture of the E. coli protein by the antibodies on the plate, a second anti- E. coli HCP antibody, conjugated with biotin, is added and incubated to allow it to bind to the captured E. coli proteins. After incubation, the plate is washed and a Streptavidin-HRP conjugate is added and incubated for 30 min. The Streptavidin-HRP conjugate will be captured by any biotin labeled antibody bound to the plate. Following a wash step to remove unbound conjugate, TMB substrate is added and is converted by the captured HRP to a colored product in proportion to the amount of HCP bound to the plate. After a short incubation to allow color development, the reaction is stopped and the intensity of the generated color is detected in a spectrophotometer plate reader capable of measuring 450nm wavelength.
E. coli HCP ELISA Kit (host cell protein) ab240997 protocol summary
1. Add samples or standards to the wells
2. Incubte 90 min then wash
3. Add reporting antibody to the wells
4. Incubate 45 min then wash
5. Add the streptavidin-HRP solution to the wells
6. Incubate 30 min then wash
7. Add TMB substrate solution - wait 10 min
8. Add stop solution and read OD at 450nm
Related and recommended products
E. coli HCP ELISA Kit (host cell protein) ab240997 is commonly used to measure E. coli during the manufacturing of biopharmaceutical production.
Other ELISA kits for measuring proteins in the development of safe biopharmaceuticals are:
- Human Albumin ELISA Kit
ab227933
- Human ALT ELISA Kit
ab234578
- Glutamate Dehydrogenase Activity Assay Kit
ab102527
- LDH Assay Kit
ab65393
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
