Description
Key facts
Detection method:Fluorescent,
Sample types:Saliva, Urine, Serum
Product details:
The Albumin Creatinine Ratio Assay Kit (ab241018) provides a simple, sensitive, and high throughput adaptable assay that detects albumin (detection range: 0.02- 2.5 mg/mL), creatinine (detection range: 0.002 -0.5 mg/mL) and Albumin-to-creatinine ratio.
The ACR ratio is determined in two steps: First, albumin is determined by using a probe (Albumin Blue 580) that specifically recognizes albumin (Ex/Em = 600/630 nm). Second, creatinine is converted to sarcosine via enzymatic reactions. Arcosine is specifically oxidized generating a product that reacts with a probe producing a chromophore that can be detected at 570 nm.
Other Notes
This product was previously called K551 Biovision Albumin-to-Creatinine Ratio (ACR) Kit. Biovision was acquired by Abcam in 2021.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin/Creatinine often abbreviated as the albumin to creatinine ratio (ACR) serves as an important marker in the body. Albumin is a plasma protein with a molecular weight of approximately 66.5 kDa. It is synthesized in the liver and widely expressed in the blood while creatinine a breakdown product of creatine phosphate is a small molecule produced in the muscles and excreted in urine. The albumin/creatinine ratio measures these levels in urine to assess excretion balance and renal function.
Biological function summary
The albumin and creatinine levels help evaluate kidney function and filtration efficiency. Albumin signifies the presence of larger proteins in urine when normal kidney filtering mechanisms are impaired. The presence and concentration of creatinine meanwhile reflect waste removal efficacy. The albumin/creatinine ratio provides a reliable estimation of microalbuminuria which is an elevation in urinary albumin considered indicative of early kidney damage. This ratio is not directly part of a complex but offers insight into the normal physiological role of kidneys.
Pathways
Albumin/creatinine measurements relate primarily to renal regulation and excretory pathways. They indicate how well glomeruli the filtering units in kidneys function relative to proteins and byproducts' reabsorption or excretion. These measurements tie to pathways involving renin-angiotensin-aldosterone system and vasopressin signaling with contacts to proteins like nephrin and podocin that maintain the integrity of the glomerular filtration barrier.
Albumin/creatinine ratios possess strong correlations with conditions such as diabetes and hypertension. High levels of this ratio suggest an increased risk for diabetic nephropathy where persistent albuminuria develops as the disease progresses. In hypertension albuminuria signifies the strain and damage on kidney structures often exacerbating chronic kidney disease. This ratio is additionally linked with angiotensin-converting enzyme (ACE) frequently altered in these conditions to manage blood pressure and minimize kidney damage.
