Description
Key facts
Sample types:Purified protein
Product details:
Albumin Depletion Kit (ab241023) provides easy and user-friendly spin columns for the depletion/removal of abundant albumin protein from samples thus enhancing the sample analysis. The kit is based on the interaction of Cibacron Blue 3G-A (immobilized on beads) with albumin. Each spin column can bind ~2-3 mg of albumin and processes samples in less than one hour. After albumin depletion, samples can be used for subsequent ELISA, immunoassays and other downstream analyses such as gel electrophoresis and functional assays. These columns can also be used for purification and enrichment of a variety of Cibacron Blue dye-binding proteins.
Please note, this kit has only been tested on human samples.
This product is manufactured by BioVision, an Abcam company and was previously called K6573 EZAlbumin™ Depletion Kit (Spin Column). K6573-25 is the same size as the 25 test size of ab241023.
The Safety Datasheet for this product has been updated for certain countries. Please check the current version in the Support and downloads section.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Albumin also known as ALB is a major plasma protein with a molecular mass of approximately 66.5 kDa. It predominantly gets synthesized in the liver and found abundantly in blood plasma. Albumin serves multiple functions including maintaining osmotic pressure and acting as a carrier protein for various endogenous substances like hormones fatty acids and bilirubin.
Biological function summary
Albumin plays a critical role in transporting small molecules like metabolites and drugs through the circulatory system. It is not a part of any complex but functions independently to bind transport and release various ligands. Albumin's affinity for hydrophobic molecules makes it essential for they to be solubilized in blood for proper metabolism and excretion.
Pathways
More than five binding sites on albumin facilitate its role in the fatty acid metabolism and renin-angiotensin system pathways. In fatty acid metabolism albumin interacts with transport proteins carrying fatty acids to tissues for energy production. Additionally during the renin-angiotensin system albumin modulates blood pressure through its interaction with angiotensin II which can influence vasoconstriction.
Researchers link albumin with nephrotic syndrome and liver cirrhosis. In nephrotic syndrome low levels of albumin (albumin low) can cause edema due to imbalanced osmotic pressure. In liver cirrhosis a reduction in albumin synthesis signifies liver damage. Albumin's interaction with proteins like angiotensin II correlates with complications such as hypertension further establishing the protein's role in health and disease contexts.
