Description
Key facts
Sample types:Tissue sections
Product details:
Bielschowsky Silver Stain Kit ab245877 is designed for histological visualization of nerve fibers, neurofibrillary tangles and senile plaques in Alzheimer's disease.
Staining Interpretation
Axons: Black
Neurofibrillary Tangles: Black
Senile Plaques: Black
Nuclei: Dark Brown
Background: Yellow to Light Brown
Control Tissue
Cerebral cortex (cut 8-10μm)
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-Multi, Storage information-Please refer to protocols
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Nerve fibers neurofibrillary tangles and senile plaques are key elements in the study of neurodegenerative diseases. Neurofibrillary tangles are intracellular aggregates of hyperphosphorylated tau proteins while senile plaques are extracellular deposits primarily composed of amyloid-beta peptides. Both structures are expressed extensively in the brain especially in regions related to cognitive functions. The precise mass of these targets varies due to their complex aggregate nature and involvement in paired helical filaments and other abnormalities. Researchers often use Gallyas silver stain and Bodian stain to visualize these structures in histological sections allowing detailed examination of their distribution and association in neural tissue.
Biological function summary
Nerve fibers neurofibrillary tangles and senile plaques significantly affect cell health. Neurofibrillary tangles disrupt cellular function by impairing microtubule stability critical for maintaining neuronal shapes and intracellular transport. Senile plaques lead to chronic inflammation and neuronal damage due to amyloid-beta aggregation which disrupts cell signaling. These structures interact with various cellular complexes including synaptic machinery and inflammatory pathways. Accumulation of these pathological features contributes to neural cell dysfunction and eventual tissue atrophy in affected brain areas.
Pathways
Nerve fibers neurofibrillary tangles and senile plaques take part in critical signaling cascades such as the amyloidogenic pathway and tau phosphorylation pathway. These targets are related to glycogen synthase kinase-3 beta (GSK3β) and beta-secretase (BACE1) proteins that regulate the phosphorylation of tau and the cleavage of amyloid precursor protein (APP) respectively. Their dysregulation alters the normal pathways contributing to disease progression and neurodegeneration.
Nerve fibers neurofibrillary tangles and senile plaques are notably associated with Alzheimer's disease and frontotemporal dementia. In Alzheimer's disease excessive amyloid-beta production and tau hyperphosphorylation lead to synaptic disturbance and neuronal loss. Frontotemporal dementia often involves a similar pathological mechanism primarily involving tau proteinopathies. These conditions share linked proteins such as APP tau and apolipoprotein E (ApoE) all inadequate in their regulation and processing within this pathological context.
