Description
Key facts
Sample types:Tissue sections
Product details:
How the assay works
Movat Pentachrome Stain Kit (Modified Russell-Movat) is particularly useful when studying the heart, blood vessels and various vascular diseases.
Elastic Fibers: Black to Blue/Black
Nuclei: Blue/Black
Collagen: Yellow
Reticular Fibers: Yellow
Mucin: Bright Blue
Fibrin: Bright Red
Muscle: Red
Movat Pentachrome Stain Kit (Modified Russell-Movat) protocol summary
- Entire tissue is initially overstained with a Verhoeff-type working Elastic Stain Solution which contains unoxidized Hematoxylin, an oxidizer (Ferric Chloride) and a mordant (Iodine).
- Excess elastin stain is then removed from tissue using a dilute Ferric Chloride solution that differentiates elastin and nuclei (black) from the rest of the tissue.
- Alcian blue, a copper phthalocyanine dye, is then used to bind acid mucins providing bright blue color.
- A trichrome-type of staining using Biebrich Scarlet/Acid, Phosphotungstic Acid, and a proprietary Yellow Stain Solution, is then employed giving red muscle and yellow collagen.
Properties and Storage Information:
Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions-Ambient, Appropriate long-term storage conditions-Ambient, Storage information-Ambient
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Movat Pentachrome Staining also known as Movat's stain is a distinctive histological staining technique designed to visualize various tissue components in a single tissue section. Mechanically Movat Pentachrome Staining highlights elements such as collagen elastin muscle and mucin. This comprehensive staining technique employs dyes that bind to specific macromolecules within the tissues allowing clear differentiation. Many laboratories use it to analyze connective tissue structure in biological samples. The stain enables clear visualization of tissue architecture particularly useful in vascular and connective tissue studies.
Biological function summary
Movat Pentachrome Staining offers insights into the complex structure of tissue samples by identifying different components within the extracellular matrix. By staining collagen and elastin with distinct colors the stain aids in analyzing structural integrity and tissue remodeling. It is not specific to a protein complex but provides an overall assessment of tissue architecture and pathology. Researchers often apply it to understand the composition and changes in tissue components in various biological contexts.
Pathways
Interactions within connective tissue matrices can be better understood using Movat Pentachrome in studies of structural remodeling. It allows exploration of pathways such as the extracellular matrix (ECM) organization and integrin signaling pathways. The ability to differentiate collagen from elastin facilitates the study of proteins like collagenases and elastases which play roles in ECM degradation and remodeling. Such pathways are essential in processes like wound healing and tissue development where changes in connective tissue structure are critical.
Movat Pentachrome Staining plays a role in diagnosing cardiovascular and connective tissue diseases. It helps characterize vascular pathologies such as atherosclerosis by clearly displaying arterial wall components. Additionally the stain assists in examining diseases like fibrosis where excess collagen deposition is a concern. Through its ability to visualize tissue components it connects to proteins like matrix metalloproteinases which are involved in the progression of these diseases by influencing extracellular matrix turnover and tissue integrity.
