Description
Key facts
Detection method:Colorimetric,
Sample types:Citrate plasma, Heparin Plasma, Serum,
Reacts with:Human,
Assay type:Sandwich (qualitative),
Assay Platform:Pre-coated microplate (12 x 8 well strips)
Product details:
Aspergillus fumigatus
IgM ELISA kit (ab247191) is designed for the qualitative determination of IgM class antibodies against
Aspergillus fumigat
us in human serum or plasma (citrate, heparin).
The qualitative immunoenzymatic determination of specific antibodies is based on the ELISA (Enzyme-linked Immunosorbent Assay) technique. Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.
Aspergillus fumigatus
is a fungus of the genus Aspergillus and is one of the most common Aspergillus species to cause disease in individuals with an immunodeficiency.
A. fumigatus
, a saprotroph, is widespread in nature and is typically found in soil and decaying organic matter. Colonies of the fungus produce thousands of small conidia (2-3 μm) that readily become airborne. The fungus is capable of growth at temperatures up to 50 °C, with conidia surviving at 70 °C. Humans are continuously in contact with these asexual spores and it is estimated that an individual inhales several hundred conidia each day; typically, these are quickly eliminated by the immune system. Aspergillosis develops mainly in individuals who are immunocompromised, either from disease or from immunosuppressive drugs, and is a leading cause of death in acute leukemia and hematopoietic stem cell transplantation.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Aspergillus fumigatus IgM also known as A. fumigatus IgM is an antibody class that recognizes antigens from the fungal organism Aspergillus fumigatus. This fungus is a common mold found in the environment and the IgM antibody is an early immune response component produced by B cells. The molecular mass of IgM is approximately 900 kDa and it is expressed mainly in the blood and lymphatic systems. The early detection of Aspergillus antigens by IgM can help initiate a protective immune response against the fungal infection.
Biological function summary
The IgM antibodies play a important role in the immune defense against Aspergillus fumigatus. When the fungal spores are inhaled IgM captures these antigens triggering the classical pathway of complement activation. This process involves multiple proteins and leads to the opsonization and phagocytosis of fungal spores improving their clearance by the immune cells. IgM does not typically exist in complex but functions efficiently to activate other immune processes.
Pathways
The activation of the classical complement pathway is a significant function of IgM. This pathway involves components such as C1q which binds to the IgM-antibody complexes and initiates the cascade leading to cell lysis or opsonization. Besides the complement system IgM is linked with various Fc receptors on immune cells which allows these cells to recognize and respond to pathogens more efficiently. The relationship between IgM and other immunoglobulin classes also highlights its role in shaping the adaptive immune response.
Aspergillus fumigatus IgM plays an important role in early defense against invasive aspergillosis a serious infection mostly affecting immunocompromised individuals. It is also relevant in allergic bronchopulmonary aspergillosis (ABPA) a hypersensitivity reaction due to Aspergillus colonization in the lungs. In these conditions the production of IgM can serve as an indicator of immune activity and potential disease diagnosis. Moreover proteins like IgE are also involved especially in allergic responses such as ABPA showing how IgM works alongside other immune components to combat fungal infections.
