Description
Key facts
Detection method:Colorimetric,
Sample types:Cell Lysate,
Reacts with:Mouse, Human,
Assay type:Semi-quantitative,
Sensitivity:< 1 µg/mL,
Range:0.05 - 3.12 %,
Assay time:1h 30m,
Assay Platform:Microplate
Product details:
AKT 1/2/3 Total (ab253298) is a single-wash 90 min sandwich ELISA designed for the semi-quantitative measurement of total AKT 1/2/3 protein in human and mouse cell lysates. It uses our proprietary SimpleStep ELISA® technology. Semi-quantitate total AKT 1/2/3 with 1 μg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
AKT isoforms belong to a conserved family of serine/threonine protein kinases. AKT plays a central role in cell survival and inhibiting apoptosis, by phosphorylating and inactivating several targets. AKT is activated by insulin and various other agonists, which promote transport to the plasma membrane, where the activation loop of AKT is phosphorylated at Thr308 by PDK1. The carboxy terminus is phosphorylated at Ser473 by mammalian target of rapamycin complex 2 (TORC2).
AKT substrates include Bad, forkhead transcription factors, and caspase-9. Another vital AKT function is the regulation of glycogen synthesis through phosphorylation of GSK-3. AKT phosphorylation also prevents GSK-3β mediated phosphorylation and degradation of cyclin D1, and negatively regulates the cyclin dependent kinase inhibitors p27 Kip and p21 Waf1/CIP1. AKT also plays a critical role in the regulation of TORC1 and tuberin (TSC2).
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AKT proteins also known as protein kinase B (PKB) include AKT1 AKT2 and AKT3 playing key roles in cellular processes. These serine/threonine kinases have molecular masses ranging between 55 to 60 kDa. AKT protein family expresses widely in multiple tissues including muscle liver and brain. Each isoform has specific but overlapping roles in cell signaling. AKT kinases often studied with ELISA kits facilitate insight into essential cellular regulation mechanisms.
Biological function summary
AKT proteins drive processes like cell survival growth and proliferation. They interact with various proteins forming complexes that regulate pathways contributing to these cellular functions. AKT activation occurs in response to signals from growth factor receptors leading to downstream effects such as protein synthesis and glycogen metabolism. These proteins also exhibit regulatory influence in apoptosis contributing to their extensive role in maintaining cellular integrity.
Pathways
AKT proteins integrate into major signaling networks. The PI3K-AKT pathway is a critical route that bounces signals from the cell membrane to regulate cell survival and growth. AKT interacts with mTOR an important component of cell growth and metabolism pathways establishing a cross-talk between them. This convergence serves as a regulatory hub orchestrating numerous downstream effects affecting cellular functions.
AKT proteins have notable implications in cancer and diabetes. Dysregulation of AKT activity associates with oncogenesis through alterations in cell proliferation and survival pathways. Moreover AKT2 plays a significant role in glucose metabolism connecting it to insulin signaling and diabetes development. These proteins also interact with PTEN a tumor suppressor affecting pathways important for understanding their roles in disease pathology.
