Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GAL, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Galanin also known as the galanin peptide is a neuropeptide that plays a role in various physiological processes. It has a molecular mass of approximately 3 kDa and is expressed widely throughout the central and peripheral nervous systems. Galanin is extensively distributed in the brain spinal cord and gut indicating its involvement in multiple neural functions.
Biological function summary
Galanin influences neurotransmitter release and modulates synaptic transmission. It often forms part of a receptor complex with galanin receptors GALR1 GALR2 and GALR3 which mediate its effects. Galanin exerts diverse actions including regulation of mood feeding behavior and modulation of nociception. These broad biological roles highlight its importance in maintaining homeostasis.
Pathways
Galanin takes part in the regulation of several key signaling pathways such as the MAPK/ERK and the cAMP-PKA pathways. It interacts with other neuropeptides and its activity can modulate the action of neurotransmitters like norepinephrine and serotonin which influence mood and stress response. These pathways reveal its integrative role in neurophysiological regulation and its connection with multiple proteins.
Galanin is linked with conditions such as depression and epilepsy. Changes in galanin expression have been observed in depressive disorders where it may interact with serotonin transporter proteins affecting mood regulation. In epilepsy galanin has a potential role in reducing seizure susceptibility possibly in relation to its interaction with the gamma-aminobutyric acid (GABA) system. Understanding these associations aids in exploring galanin as a potential therapeutic target.
