Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HINT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HINT1 also known as Histidine Triad Nucleotide-Binding Protein 1 is a hydrolyzing enzyme with a monomeric mass of approximately 14 kDa. It functions mechanically by catalyzing the phosphoramidate bond in nucleotides which releases free phosphates. HINT1 is part of the family of histidine triad proteins and is expressed broadly across various tissues including the brain heart and testis indicating its potential importance in those areas.
Biological function summary
Within cellular processes HINT1 plays a role as a transcriptional regulator especially influencing the p53 pathway and those involved in stress responses. It forms part of a complex with other proteins guiding cellular responses to DNA damage. This activity strongly suggests HINT1's role in maintaining genomic stability and influencing cell cycle checkpoints.
Pathways
Several important signaling cascades involve HINT1 such as p53 and MAPK pathways. In the context of the p53 pathway HINT1 enhances tumor suppression activities by regulating transcription and apoptosis processes. Through the MAPK pathway it influences cell proliferation and apoptosis. Other proteins like p53 itself and c-Myc closely interact with HINT1 within these signaling pathways coordinating responses to cellular stress and growth signals.
There is a strong association between HINT1 and cancer as well as neuropathic disorders. Its role in tumor suppression suggests that dysfunction or altered expression may contribute to cancer progression particularly cancers related to the lung and colon. Moreover studies indicate a link between HINT1 and schizophrenia where alterations in protein expression can affect neural signaling. Proteins such as p53 and NMDAR which are altered in these disorders connect to HINT1 further displaying its significance in the development and progression of these diseases.
