Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 2 and 4 bp deletion in exon 2.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ARF3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ADP-ribosylation factor 3 commonly known as ARF3 is a small GTP-binding protein with a mass of approximately 20 kDa. It belongs to the ARF family and is expressed in many tissues especially within cellular compartments that are highly dynamic including the Golgi apparatus and the cytoplasm. ARF3 functions by binding and hydrolyzing GTP which is an important function in regulating vesicular trafficking and membrane lipid metabolism.
Biological function summary
The functions performed by ARF3 revolve around the regulation of vesicle formation and trafficking within cells. It is critical in the formation of COPI-coated vesicles and is involved in the retrieval pathway of membrane proteins between the Golgi and endoplasmic reticulum. ARF3 interacts closely with proteins like coatomer and ARF-GEFs (guanine nucleotide exchange factors) to facilitate these processes. As part of its biological activity ARF3 affects the recruitment of various effector proteins necessary for the vesicular transport.
Pathways
ARF3 plays significant roles in the regulation of intracellular transport pathways and lipid signaling pathways. It interacts with proteins involved in the phosphatidylinositol signaling pathway influencing membrane dynamics and cell signaling. ARF3's function aligns it closely with proteins such as phosphatidylinositol 4-kinases which are key in modifying phosphoinositides that are essential for membrane trafficking and signal transduction.
Connections between ARF3 and pathological conditions like cancer and neurodegenerative diseases have been observed. Abnormal expression or mutation of ARF3 may lead to disruptions in membrane trafficking contributing to disease progression. ARF3 has shown links with proteins such as RAB proteins in the context of cancer as they collaboratively affect cellular processes relevant to tumor progression such as cellular migration and invasion.
