Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon1 and 1 bp insertion in exon1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ARF5, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ADP-ribosylation factor 5 (ARF5) protein also known by its primary name is an important part of the ARF family. It has a mass of around 20 kDa. ARF5 plays a role in regulating vesicle trafficking systems particularly linked with the Golgi apparatus. It is found broadly expressed in numerous tissues with high levels observed in the brain and testes. This small GTP-binding protein is associated with intracellular signaling cascades and cellular communication.
Biological function summary
ARF5 is involved in modulating key cellular processes including membrane trafficking and lipid metabolism. It directly participates in the formation of vesicle coats by aiding the recruitment of coat protein complexes. ARF5 also interacts within cellular complexes impacting the transport of cargo in the secretory pathway. It also affects actin cytoskeleton remodeling thereby influencing cell shape and motility.
Pathways
The ARF5 protein contributes to both the COPI and clathrin-coated vesicle formation pathways. It functions within intricate signaling networks coordinating with other small GTPases such as ARF1. ARF5's activity links to pivotal roles within these pathways affecting protein and lipid sorting. These pathways are vital for maintaining cellular homeostasis and facilitating effective intracellular transport.
ARF5's dysfunction has been linked to neurodegenerative disorders including Alzheimer's disease. Variations in the expression or mutations of ARF5 might contribute to the disease pathology by impairing vesicular transport and potentially interacting with proteins like tau. Furthermore ARF5 may relate to metabolic syndromes due to its involvement in lipid metabolism with implications for body fat distribution and lipid-related diseases.
