Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-DRG1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The DRG1 protein also known as Developmentally Regulated GTP-binding protein 1 is an important player in cellular functions. This highly conserved protein has a mass of approximately 42 kDa and is expressed in various tissues including the brain and testis indicating its essential role in different biological processes. It contains GTP-binding motifs important for its activity which allows the protein to interact with other molecular partners in the cell facilitating its participation in cellular events.
Biological function summary
DRG1 engages in several critical cellular processes beyond its mechanical functions. One of its notable roles involves contributions to ribosome biogenesis although it does not form a permanent complex with the ribosome. The protein supports cellular growth and division highlighting its potential in affecting cell cycle regulation. DRG1 associates transiently with other proteins allowing it to influence a range of biological functions including cellular growth signaling pathways.
Pathways
Research indicates that DRG1 links to important regulatory networks such as the Ras signaling and translational control pathways. Within these pathways DRG1 interacts with proteins like eukaryotic initiation factors which are important for translation regulation. This interaction plays a role in coordinating protein synthesis which supports overall cellular growth and response to environmental cues. Understanding its place in these pathways sheds light on how cells control protein production and growth.
Studies suggest DRG1's involvement in cancer and neurological disorders. In certain cancers altered expression of DRG1 correlates with tumor progression implicating its role in cell proliferation and survival mechanisms. Additionally DRG1 has connections to neurodegenerative disorders potentially through interactions with tau protein which is associated with abnormal protein deposits in the brain. The protein’s link to these diseases highlights the need for further research into its functional mechanisms and potential as a therapeutic target.
