Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FZD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Frizzled homolog 1 (FZD1) also known as Fz-1 is a member of the Frizzled family of G protein-coupled receptor proteins involved in the Wnt signaling pathway. FZD1 has a molecular mass of approximately 74 kDa. It consists of seven transmembrane domains and is found mainly on the cell surface. Expression of FZD1 occurs in various tissues including the kidney heart liver and lung. The target acts as a receptor for Wnt proteins facilitating the binding and signaling required for downstream effects.
Biological function summary
FZD1 participates in important processes concerning cell development differentiation and growth. As a member of the Frizzled family FZD1 often forms a functional complex with LRP5/6 co-receptors upon Wnt ligand binding. This complex initiates intracellular signaling cascades that lead to the regulation of target genes. In addition to its role in development FZD1 also has a major impact on maintaining stem cell renewal and tissue homeostasis.
Pathways
FZD1 functions significantly in both the canonical and non-canonical Wnt signaling pathways. In the canonical pathway FZD1 interacts with proteins such as Disheveled (DVL) and β-catenin leading to alterations in gene expression that influence cellular proliferation and differentiation. In the non-canonical pathway FZD1 also associates with other proteins including Rho and JNK which contribute to changes in cell polarity and motility. These pathways highlight FZD1's involvement in complex networks essential for cellular and tissue dynamics.
FZD1 has associations with conditions like cancer and osteoporosis. Aberrations in FZD1 expression or function can lead to disrupted Wnt signaling contributing to tumorigenesis by affecting cell proliferation and survival pathways. FZD1's role in bone development ties it to osteoporosis where improper signaling affects bone density and strength. Moreover FZD1 associates with other proteins such as DVL and β-catenin in these pathological processes highlighting its impact on disease progression.
