Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GIT1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GIT1 also known as G protein-coupled receptor kinase interacting protein 1 is a multi-domain protein with a mass of approximately 90 kDa. It plays a central role in cellular signaling processes. GIT1 contains a GTPase-activating protein (GAP) domain and a number of other domains that interact with protein partners. This protein is expressed ubiquitously in various tissues with higher expression levels in the brain and heart. Its presence in different cell types indicates its involvement in numerous cellular functions.
Biological function summary
GIT1 interacts with several proteins to form multi-protein complexes essential for cellular dynamics. It functions in regulating the actin cytoskeleton impacting cell adhesion migration and membrane trafficking. GIT1 participates in the reorganization of the cytoskeleton by activating small GTPases which are important for these processes. Additionally it interacts with paxillin and ARF GTPases further playing a role in focal adhesion signaling.
Pathways
GIT1 serves an important role in the ERK and JNK signaling pathways. It acts as a scaffolding protein facilitating the assembly of signaling complexes that promote the transfer of signals from the membrane to the nucleus. GIT1 modulates the activity of proteins such as MEK1/2 and paxillin which ultimately regulate cellular responses like proliferation and differentiation. This function positions GIT1 at an important junction of pathways controlling cellular architecture and stress responses.
GIT1 has been linked to cardiovascular diseases and neurological disorders. Dysregulation of GIT1 and its associated pathways can lead to pathological changes in the heart contributing to conditions such as cardiac hypertrophy. In the nervous system altered GIT1 activity can affect synaptic functions associated with disorders like schizophrenia. In these contexts interactions between GIT1 and other proteins such as beta-arrestins become significant impacting disease progression and therapeutic targeting.
