Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HMGN2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HMGN2 also known as high mobility group nucleosome binding domain 2 is a small protein weighing approximately 10.1 kDa. It is a non-histone chromosomal protein that interacts directly with nucleosomes modulating chromatin structure and function. HMGN2 is widely expressed in various tissues and cells and it plays a role in gene regulation by affecting the chromatin compaction and transcriptional activity.
Biological function summary
HMGN2 influences cellular processes by binding to the nucleosome core particle within chromatin. It is a component of a dynamic chromatin complex that affects gene expression by remodeling chromatin structure making specific genomic regions more accessible to transcription machinery. HMGN2's interaction with nucleosomes can regulate a host of genes involved in growth and differentiation highlighting its role in modulating cellular functions.
Pathways
Chromatin remodeling and transcriptional regulation involve HMGN2 as a significant player. It participates in important pathways like gene expression regulation and cellular responses to DNA damage. HMGN2 interacts with proteins such as histones and other high mobility group proteins which orchestrate these pathways by modifying chromatin architecture and influencing transcription factor access.
HMGN2 shows relations to cancer and autoimmune diseases. Altered expression or mutations in HMGN2 can disrupt normal chromatin dynamics contributing to oncogenesis by deregulating genes involved in cell cycle control. Additionally autoimmune conditions may arise due to the immune system's aberrant response to misregulated HMGN2 affecting proteins such as histones that interact with this target. Understanding HMGN2's role in disease can offer insight into potential therapeutic strategies.
