Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 0 bp deletion in exon1 and 32 bp deletion in exon1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HNRNPA3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HNRPA3 also known as heterogeneous nuclear ribonucleoprotein A3 is a protein involved in RNA-related processes. It has a molecular mass of approximately 39 kDa and shows expression in various tissues with higher expression in neuronal cells. This protein plays a significant role in RNA metabolism through RNA-binding activities. HNRPA3 participates in the processing transport and stability of mRNA molecules by interacting with other RNA-binding proteins.
Biological function summary
HNRPA3 is key in regulating RNA splicing by forming complexes with components such as hnRNPs a diverse group of RNA-binding proteins. These complexes contribute to the modification and alternative splicing of pre-mRNA which is essential for generating protein diversity. HNRPA3 stabilizes mRNA and influences the transcriptional activity impacting gene expression regulation. Its interactions ensure mRNA molecules are correctly processed and available for translation.
Pathways
HNRPA3 actively participates in the spliceosome pathway which is responsible for the splicing of pre-mRNA. Additionally it plays a role in the mRNA surveillance pathway ensuring the fidelity of RNA molecules. Through these pathways HNRPA3 collaborates with proteins such as HNRNPA1 and SRSF1 which also facilitate RNA binding and processing activities. This coordination is critical for maintaining cellular homeostasis.
HNRPA3 associates with neurodegenerative diseases and certain cancers. Aberrations in HNRPA3 function can contribute to frontotemporal lobar degeneration due to mislocalization and aggregation of its protein. In cancer altered expression or mutations in HNRPA3 can disrupt normal RNA splicing leading to tumor progression. Proteins like TDP-43 and FUS which share functional domains with HNRPA3 often interact with HNRPA3 in these pathological conditions suggesting a networked dysfunction in RNA processing mechanisms.
