Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon4.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-APOL2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APOL2 also known as Apolipoprotein L2 is a protein with molecular mass about 38 kDa. This protein belongs to the apolipoprotein L family and shows expression in various tissues including the pancreas lung and placenta. APOL2 contains lipid-binding domains that enable it to interact with lipids in cellular membranes. It involves in lipid metabolism by aiding the transport of lipids through intracellular compartments.
Biological function summary
APOL2 contributes to lipid trafficking and cellular lipid homeostasis. It plays a role in the formation of lipid droplets within cells significant for energy storage and regulation. APOL2 does not function alone but often acts in coordination with other proteins involved in lipid metabolism including members of its own family. This association through protein complexes allows for efficient regulation of lipid movement and storage in cells.
Pathways
Several important pathways intersect with APOL2's functions. It participates in the lipid metabolism pathway which affects energy balance within cells. Another pathway where APOL2 plays a role is the inflammatory response pathway as its expression can influence the response to inflammatory signals. APOL2 shows functional connections with other apolipoprotein family members such as APOL1 through these pathways helping to maintain cellular biochemical equilibrium.
APOL2 relates to cancer and cardiovascular diseases. Aberrant expression of APOL2 can potentially influence cancer progression by impacting cellular lipid metabolism contributing to tumor growth. In cardiovascular diseases its role in lipid metabolism correlates with the regulation of blood lipid levels affecting cardiovascular health. Notably APOL2's function in cardiovascular diseases may interact with APOL1 which has known links to kidney-related disorders and cardiovascular risk factors.
