Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 7 bp deletion in exon1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ARL6IP1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARL6IP1 also known as ADP-ribosylation factor-like protein 6 interacting protein 1 functions mechanically as a transmembrane protein with a mass of approximately 20 kDa. It localizes principally in the endoplasmic reticulum and Golgi apparatus. Researchers have identified it in various tissues with higher expression in neural tissues. The protein plays a role in vesicular trafficking impacting membrane dynamics and protein sorting within cells.
Biological function summary
ARL6IP1 acts as a modulator of intracellular membrane structures and dynamics. It is not typically understood as part of a larger macromolecular complex but rather operates through interactions with membrane components. The protein assists in membrane curvature processes and is important in maintaining cellular compartmentalization.
Pathways
Several research studies implicate ARL6IP1 in the regulation of the vesicular transport pathway. It interacts with proteins involved in the Golgi to endoplasmic reticulum transport like the COPI machinery. ARL6IP1 also associates with lipid metabolism pathways through its regulatory roles particularly touching on relationships with proteins such as ARF1 that are active in endomembrane system organization.
Research highlights ARL6IP1's influence in disorders involving neurodegeneration. Its dysfunction associates with conditions such as hereditary spastic paraplegia a disorder characterized by progressive weakness and spasticity. Studies note its interactions with proteins like SPAST which is also involved in the pathogenesis of related neurological disorders.
