Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon2 and 8 bp deletion in exon2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-DENND5A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
DENND5A also known as connecdenn 5 serves as a guanine nucleotide exchange factor (GEF). It regulates the activation of small GTPase Rab-GDP to Rab-GTP which is involved in vesicular trafficking processes. The protein has a mass of approximately 127 kDa. DENND5A expression shows presence in many tissues including the brain pancreas and kidney indicating its diverse functional roles across different organ systems.
Biological function summary
DENND5A influences intracellular signaling and membrane trafficking. It does this by activating Rab protein family members essential for vesicle formation movement and fusion. DENND5A participates in endocytic pathways which involves clathrin-mediated endocytosis a pathway critical for material uptake into cells. There is no clear evidence yet whether DENND5A associates within larger protein complexes but its interaction with Rab proteins suggests close collaboration in cellular trafficking tasks.
Pathways
DENND5A integrates into the membrane trafficking and cellular communication networks. It plays an important role in the endocytosis pathway and syncs with proteins like Rab22A facilitating efficient vesicular transport from the plasma membrane. Another significant pathway is the mannose 6-phosphate receptor pathway where DENND5A influences lysosomal enzyme trafficking vital for cell maintenance.
DENND5A mutations or dysregulations link to neurodevelopmental conditions like epilepsy and intellectual disability. These associations suggest its importance in neural cell signaling. DENND5A itself indirectly interacts with proteins such as RAB18 which are implicated in Warburg Micro syndrome a rare genetic disorder affecting eye and brain development. Through these interactions its role in disease pathogenesis becomes more apparent highlighting the potential for targeted therapeutic strategies.
