Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FAM98A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FAM98A also known as Family with Sequence Similarity 98 Member A is a cytoplasmic protein with a mass of approximately 26 kDa. This protein shows expression in various tissues including the brain and liver. It functions mechanically in processes involving RNA contributing to RNA-binding activities although the specific details of its RNA interactions remain to be fully understood.
Biological function summary
This protein acts within a larger multi-protein complex affecting cellular and molecular functions that are important for proper cell regulation. It participates in processes like gene expression modulation although the precise scope of its biological roles is still under investigation. FAM98A also appears to engage in interactions influencing cell cycle regulation which highlights its importance in maintaining cell stability.
Pathways
FAM98A integrates into significant cellular pathways such as TOR signaling and may also influence mitotic processes. It interacts with proteins like the RACK1 scaffolding protein suggesting a role in protein translation and signaling pathways. These interactions position FAM98A within important circuits that oversee cell growth and proliferation.
Changes in FAM98A function or expression could contribute to cancer and neurological disorders reflecting its involvement in cell cycle and regulatory processes. For instance in certain cancers its altered interaction with proteins like RACK1 may impact tumor progression. Meanwhile in neurological disorders disruptions in its RNA-binding role might link to dysregulation of neuronal functions. Further research could open additional insights into these associations.
