Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 2 bp deletion in exon4.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-INVS, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Inversin also known as INV or INVS is a protein that plays a mechanical role in cellular signaling particularly in the process of cilia assembly and maintenance. It has a molecular weight of approximately 124 kDa. This protein is expressed in various tissues with notable abundance in the kidneys and other ciliated cells. Inversin is important for the function and structure of cells contributing to their ability to sense and respond to environmental cues.
Biological function summary
Inversin acts as an essential modulator of cell-cycle regulation and planar cell polarity. This protein is part of a complex that regulates the Wnt signaling pathway acting as a switch between canonical and non-canonical Wnt pathways. Inversin's role ensures the proper orientation and division of cells which is necessary for correct tissue development and function. In this way Inversin influences cell and organ structure and development.
Pathways
Inversin functions in the Wnt signaling pathway and the Hedgehog signaling pathway. In the Wnt pathway it interacts with Dishevelled proteins modulating pathways to ensure proper signaling for development. In the Hedgehog pathway Inversin plays a role in coordinating signal transduction necessary for embryonic patterning and development. Proper engagement in these pathways ensures the correct relay of signals that influence numerous biological processes.
Inversin has been linked to nephronophthisis and situs inversus. Nephronophthisis a kidney disorder correlates with mutations in the inversin gene which disrupt cilia function. This dysfunction leads to kidney fibrosis and cyst formation. In addition Inversin's connection to situs inversus involves a misalignment in the left-right organization of internal organs highlighting its role in ensuring normal body asymmetry. In these diseases aberrations in proteins such as cystin and inversin contribute to improper cellular and organ function.
