Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon3 and 1 bp deletion in exon3 and 7 bp deletion in exon3.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ISM1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ISM1 protein also known as Isthmin-1 plays a significant role in cellular processes. It has a molecular mass of approximately 43 kDa. This protein is widely expressed across different tissues notably in the endothelial cells and adipose tissue. ISM1 contributes to various cellular mechanisms particularly those involving angiogenesis and cell differentiation. Understanding the mechanical actions of ISM1 offers insights into its diverse roles in human physiology.
Biological function summary
ISM1 functions in angiogenesis and is involved in controlling cell proliferation and apoptosis. It does not form part of a protein complex but interacts with other molecules to regulate these processes. In addition ISM1 can influence the modulation of immune responses and exhibits potential anti-inflammatory properties. This biological activity suggests that ISM1 is significant for tissue development and homeostasis.
Pathways
ISM1 integrates into the angiogenesis and apoptotic pathways. In the angiogenesis pathway it interacts with proteins like VEGFR2 which leads to the promotion of new blood vessel formation. Furthermore in the context of apoptosis ISM1 can modulate pathways involving the protein Bcl-2 which plays an important role in cell death regulation. These interactions highlight ISM1's participation in vital biological pathways that maintain cellular function and viability.
ISM1 associates with cancer and obesity. In cancer the protein's role in angiogenesis can influence tumor growth and vascularization making it a potential target for cancer therapy. ISM1 also connects to obesity through its expression in adipose tissue interacting with proteins like leptin that influence metabolism and energy homeostasis. These disease associations highlight the importance of ISM1 as a biomarker and a potential target in therapeutic interventions.
