Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-KDELR2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ELP-1 also known as Elongator complex protein 1 or KDEL receptor 2 is a protein with a molecular weight of approximately 150 kDa. This protein is an important component of the Elongator complex which is involved in transcriptional elongation by RNA polymerase II. ELP-1 is ubiquitously expressed in various tissues suggesting its significance across multiple cell types. Its presence within the Elongator complex a multi-subunit assembly facilitates the proper modification of tRNA impacting protein synthesis efficiency.
Biological function summary
ELP-1 plays an important role in facilitating the modification of tRNA wobble uridines. This allows for accurate and efficient translation of the genetic code into proteins. ELP-1 as a vital part of the Elongator complex also contributes to the acetylation of histones which impacts chromatin structure and gene expression regulation. The protein ensures that gene transcription proceeds effectively by interacting with different components of the transcription machinery.
Pathways
The Elongator complex including ELP-1 integrates into the transcriptional regulation and tRNA modification pathways. ELP-1 interacts with several proteins like RNA polymerase II during gene transcription. Another intersecting pathway includes the regulation of response to cellular stress as correct modification of tRNA maintains protein synthesis under stress conditions. Its functionality within these pathways illustrates its integral role in ensuring proper cellular response to environmental changes.
ELP-1 is linked to familial dysautonomia a neurodevelopmental disorder that affects autonomic nervous system functioning. Mutations in the IKBKAP gene encoding ELP-1 impair its function leading to the disease's manifestation. Additionally there is evidence suggesting ELP-1's involvement in multiple cancer types where altered elongator complex activity can affect tumor progression. In these contexts ELP-1's interactions with other proteins like IKBKAP become highly relevant for understanding the disease mechanisms and potentially developing therapeutic interventions.
