Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ALDH9A1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ALDH9A1 also known as aldehyde dehydrogenase 9 family member A1 is an enzyme with a molecular mass of approximately 56 kDa. It catalyzes the conversion of aldehydes to carboxylic acids using NAD+ as a cofactor. ALDH9A1 is found in various tissues including the liver brain and kidney where it plays a role in the detoxification processes and metabolism of aldehydes which can be toxic to cells if not properly managed.
Biological function summary
Aldehyde dehydrogenase 9 family member A1 contributes to cellular homeostasis and plays a protective role by processing aldehydes derived from alcohol metabolism and lipid peroxidation. It forms part of the larger aldehyde dehydrogenase family but it does not appear to engage in complex formations with other proteins in any significant manner. ALDH9A1 also assists in the degradation of histidine and proline amino acids that require processing for proper cellular function.
Pathways
ALDH9A1 is significantly involved in the metabolic pathways of amino acids and fatty acids. In these pathways it is closely related to other aldehyde dehydrogenase proteins like ALDH2 which is active in metabolizing acetaldehyde within the alcohol metabolism pathway. Both ALDH9A1 and these proteins help maintain the balance of aldehyde concentrations ensuring no cellular damage occurs due to aldehyde accumulation.
ALDH9A1 has been implicated in neurological conditions such as Parkinson's disease due to its role in preventing aldehyde-induced oxidative stress. Additionally ALDH9A1 may be relevant to certain types of cancers where aldehyde metabolism disrupts normal cellular functions. Interestingly its relation to these diseases often connects it to proteins like ALDH2 which has similar detoxification roles. Understanding its interactions and contributions in these contexts remains an ongoing area of research.
