Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 4 bp deletion in exon1 and 88 bp insertion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-BRK1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C3orf10 also known as ARMH4 or Armadillo Repeat Containing 4 encodes a protein with a currently limited mechanical description. This protein weighs approximately 54 kDa and is expressed in multiple tissues notably in the brain and skeletal muscle. Studies indicate it may play a role in cellular architecture or signal transduction but exact mechanical roles require further investigation.
Biological function summary
The protein product of C3orf10 may participate in cellular structural organization or intracellular signaling. Evidence suggests it functions as part of a larger protein complex though not extensively characterized. It possibly assists in linking cellular components to ensure structural integrity or facilitate signaling processes in cells.
Pathways
C3orf10 integrates into certain cellular pathways potentially involving cytoskeleton organization or signaling cascades. It associates with proteins involved in structural support and signal relay including some members of the armadillo family known for roles in cell adhesion and signaling. However the precise pathways C3orf10 participates in remain to be fully elucidated.
C3orf10 holds interest in research related to neurodegenerative conditions and muscular disorders. Alterations in its expression or function may link to conditions like amyotrophic lateral sclerosis (ALS) or dystrophin-related muscle dysfunction. C3orf10 could interact with proteins such as dystrophin or others involved in neurodegeneration highlighting its potential impact on disease pathways.
