Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-BRWD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The WDR9/BRWD1 protein is also known by its alternate name BRWD1 and has a molecular mass of approximately 250 kDa. This protein shows expression in various tissues with higher levels observed in testis and certain brain regions. WDR9/BRWD1 is a member of the WD-repeat protein family and contains two bromodomains. Mechanically it plays a role in mediating protein-protein interactions aiding in chromatin organization and gene regulation.
Biological function summary
WDR9/BRWD1 functions in regulating transcriptional processes and is often part of nuclear chromatin complexes. It participates in gene expression important for spermatogenesis and neuronal differentiation. This protein interacts with other chromatin-modifying proteins contributing to the alteration of chromatin states and affecting cellular programs.
Pathways
Different regulatory and developmental processes involve WDR9/BRWD1. The protein plays roles within chromatin remodeling pathways. It engages with pathways such as histone modification and transcriptional regulation working alongside proteins like histones and other transcription factors which helps coordinate the accessibility of transcriptional machinery to DNA.
Abnormal WDR9/BRWD1 expression or mutations have connections to neurological and reproductive conditions. Studies link its dysregulation to intellectual disabilities and fertility issues. Its association with chromatin-modifying proteins implicates it in diseases where gene expression regulation plays a role such as certain cognitive disorders.
