Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon 1 and Insertion of the selection cassette in exon 1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-C12orf57, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GRCC10 also known as G-protein coupled receptor C10 is a transmembrane receptor with a molecular mass of approximately 50 kDa. This protein is expressed in various tissues including the brain liver and lungs. The receptor functions mechanically by binding to specific ligands initiating intracellular signaling cascades. The activation of GRCC10 influences numerous cell processes resulting in different physiological outcomes depending on tissue type.
Biological function summary
GRCC10 plays a significant role in cell communication and signaling. It functions as part of a larger receptor complex which allows it to effectively transmit signals from the extracellular environment to the cell's interior. This signaling affects numerous biological processes such as cell proliferation differentiation and apoptosis. GRCC10's activity is important for maintaining the balance between these cellular outcomes which is important for normal development and function.
Pathways
GRCC10 participates in important signaling networks such as the MAPK/ERK and PI3K/AKT pathways. These pathways control cell growth survival and metabolism. GRCC10 interacts with proteins like RAF and AKT within these pathways helping to modulate their activity. Through its pathway involvement GRCC10 contributes to the regulation of important biological processes including response to hormones and growth factors.
GRCC10 has been linked to conditions like cancer and neurological disorders. Aberrant expression or malfunction of GRCC10 can lead to disease progression often through its interaction with proteins like p53 in cancer or tau in neurodegenerative diseases. Research continues to explore the therapeutic potential of targeting GRCC10 to modulate its role in these disorders.
