Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 50 bp deletion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-CEP57, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CEP57 also known as Centrosomal Protein 57 or Translokin is a protein with a molecular mass of approximately 57 kDa. CEP57 plays an important mechanical role in the centrosome which is important for the organization of microtubules during cell division. This protein localizes in the centrosome area within the cell cytoplasm. Expression of CEP57 is widespread occurring in various tissues which suggests its function is significant in different cellular contexts.
Biological function summary
CEP57 is important for proper cell division and spindle pole formation. It interacts within a complex of centrosomal proteins playing roles in microtubule nucleation and stabilization. CEP57 also functions as a microtubule-binding protein aiding the accurate segregation of chromosomes during mitosis. By stabilizing microtubule-kinetochore attachments CEP57 ensures effective chromosomal distribution hence maintaining genomic integrity and preventing potential cellular abnormalities.
Pathways
CEP57 contributes significantly to the cell cycle and mitotic spindle organization pathways. It works alongside other centrosomal proteins such as CEP192 and γ-tubulin enhancing their interaction and stabilization of microtubules. CEP57 is also linked to the microtubule-organizing center integrating into pathways important for cell division and duplication processes which are central to maintaining cellular homeostasis and function.
Researchers have associated CEP57 with certain cancers and mosaic variegated aneuploidy syndrome. In cancer overexpression or altered function of CEP57 might lead to improper cell division contributing to tumorigenesis. Furthermore in mosaic variegated aneuploidy syndrome improper function of CEP57 leads to chromosomal instability and abnormal cell growth. CEP57 involvement in these diseases highlights its interactions with other proteins such as TP53 which also manages cell cycle regulation and genomic stability.
