Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-EMC8, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein COX4NB also known as Cytochrome C Oxidase Subunit 4 Neighbor has a mass of around 20 kDa. It plays a mechanical role closely related to the function of its neighboring COX4 isoform proteins which are part of the cytochrome C oxidase complex. COX4NB is expressed in various human tissues including the heart and skeletal muscle suggesting its involvement in energy metabolism.
Biological function summary
COX4NB participates in mitochondrial function. The protein integrates into the cytochrome C oxidase complex which is an important component of the electron transport chain in mitochondria. Through this involvement COX4NB contributes to cellular respiration and ATP production which maintain energy homeostasis in cells.
Pathways
COX4NB is involved in key metabolic pathways. It is part of the oxidative phosphorylation pathway which is essential for the generation of ATP in aerobic organisms. In this process COX4NB works alongside proteins like COX4I1 and other subunits of the cytochrome C oxidase complex to facilitate efficient electron transfer and energy production.
COX4NB has associations with conditions tied to mitochondrial dysfunction. Its altered expression or mutation could relate to mitochondrial cardiomyopathy or hypertrophic cardiomyopathy conditions that affect heart muscle efficiency. Researchers are studying the link between COX4NB and proteins like COX4I1 as changes in these proteins could illuminate pathological mechanisms underpinning such mitochondrial diseases.
