Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 2.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FAM120A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FAM120A also known as OSSA is a protein that plays a role in cellular signaling. It has a mass of approximately 120 kDa. Researchers observe the expression of this protein in various tissues including testis ovary and brain. FAM120A functions as a scaffolding protein which means it helps organize other proteins at specific locations within cells to facilitate interactions and signaling pathways.
Biological function summary
FAM120A interacts with numerous cellular components and exhibits roles in regulating stress response. It integrates into the RNA granule complex allowing it to influence RNA stability and translation. Through its interaction with the cytoplasmic polyadenylation element-binding proteins it modulates mRNA processing offering insight into how cells manage protein production in response to environmental changes.
Pathways
FAM120A becomes integrated into signaling cascades like the PI3K/Akt pathway. It influences cell survival and growth by modulating interactions with proteins such as Src and Grb2. These interactions contribute to the regulation of key signaling activities that impact various cell functions including proliferation and apoptosis.
FAM120A has connections to cancer and cardiomyopathies. Alterations in its expression or function may affect disease pathogenesis. For instance its relationship with Src contributes to oncogenic signaling pathways influencing cancer progression. In cardiac diseases this protein interacts with pathways that involve binding partners like PI3K where its dysregulation can lead to aberrant heart function or pathology.
