Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon3.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FANCG, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FANCG also known as Protein FACG or Fanconi anemia group G protein is a component involved in DNA repair. It has a mass of approximately 68 kDa. This protein is mainly expressed in tissues with high proliferative rates such as bone marrow and germ cells. Mechanically FANCG plays an important role in the maintenance of genomic stability by participating in the repair processes that fix DNA interstrand crosslinks.
Biological function summary
Fanconi anemia group G protein is an essential part of the Fanconi anemia (FA) core complex which consists of several FA proteins. This complex is indispensable for cellular resistance to DNA damage as it repairs broken DNA strands and stalls replication forks. FANCG as part of this multiprotein complex ensures proper cellular responses to genotoxic stresses and helps in preventing mutations that could be passed to daughter cells.
Pathways
FANCG primarily functions within the FA/BRCA pathway an essential mechanism for DNA damage response and repair. It interacts closely with other FA proteins such as FANCA and FANCJ to facilitate the monoubiquitination of the FANCD2-FANCI complex a critical step in the DNA damage repair. Additionally the FA pathway is linked to homologous recombination repair where FANCG indirectly associates with BRCA1 and BRCA2 proteins coordinating with them to maintain genomic integrity.
Defects in FANCG are directly associated with Fanconi anemia a rare genetic disorder characterized by bone marrow failure and increased cancer susceptibility. FANCG mutations disrupt its interaction within the FA complex leading to increased cellular susceptibility to DNA damage. Moreover alterations in FA pathway components including FANCG also relate to specific cancer types such as acute myeloid leukemia. The protein engages in interactions with other FA pathway proteins like FANCD2 affecting disease progress and therapeutic outcomes.
