Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon3 and 1 bp insertion in exon3.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-GBP3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein known as GBP3 also called Guanylate Binding Protein 3 is a member of the guanylate-binding protein family. This protein has a molecular mass of approximately 67 kDa. It mainly functions in cellular processes involving GTP hydrolysis which plays a significant role in certain immune responses. GBP3 expresses in various tissues including the heart placenta and lungs showing higher presence in immune-related cells and tissues.
Biological function summary
GBP3 contributes to cellular defense by promoting anti-viral states and mediating immune signaling. It has been found to associate with large protein complexes that enhance its stability and activity during inflammation. This involvement supports various immune functions and contributes to the body's response to pathogenic threats. Such actions highlight GBP3's importance in maintaining cellular immunity and promoting pathogenic resistance.
Pathways
GBP3 interacts within the interferon-gamma signaling pathway where it facilitates host defense mechanisms. The protein's activity aligns closely with the JAK-STAT pathway important for the transduction of extracellular signals from the environment to the cell nucleus. GBP3 operates alongside other proteins such as GBP1 and GBP5 which are also part of guanylate-binding proteins involved in immune regulation and protection against intracellular bacteria and viruses.
Impairments in GBP3 function have connections to infectious diseases and inflammatory disorders. Studies suggest that altered GBP3 expression might relate to an increased susceptibility to viral infections such as hepatitis. Additionally abnormalities in this protein may associate with chronic inflammatory conditions like Crohn's disease. GBP3 interacts with proteins like STAT1 and GBP1 which play critical roles in modulating immune response and maintaining homeostasis within inflammatory conditions.
