Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-HMGN3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HMGN3 also known as High Mobility Group Nucleosome-Binding Domain 3 is a protein that interacts with chromatin and influences its structure. It weighs approximately 10 kDa. HMGN3 is expressed in various tissues with high levels found in the brain pancreas and kidney. This protein regulates chromatin compaction impacting gene expression and cellular functions.
Biological function summary
HMGN3 functions by modulating the access of transcription factors to DNA. It is not a part of any known multi-protein complex but acts independently. By altering the chromatin landscape HMGN3 plays a role in the regulation of genes involved in metabolism and neurodevelopment impacting cellular processes like energy balance and synaptic function.
Pathways
HMGN3 participates in the insulin signaling and neurodevelopmental pathways. It influences the insulin signaling pathway by affecting genes that manage glucose metabolism. Related proteins include insulin receptor substrate 1 (IRS1) as they work together to ensure proper metabolic responses. In the neurodevelopmental context HMGN3 interacts with pathways involving the brain-derived neurotrophic factor (BDNF) contributing to the development and plasticity of neurons.
HMGN3 relates to type 2 diabetes and neurodegenerative diseases like Alzheimer's. The role of HMGN3 in glucose metabolism links it to type 2 diabetes alongside proteins like IRS1 that influence insulin resistance. In Alzheimer's disease HMGN3 might contribute to synaptic dysfunction interacting with BDNF and other neurotrophic factors signaling potential areas of therapeutic interest.
