Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ABHD17A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
FAM108A1 also known as Family with Sequence Similarity 108 Member A1 is a poorly characterized protein with an approximate molecular mass of 27 kDa. This protein is expressed throughout various tissues with notably higher levels in neuronal tissues. The protein may possess specific motifs that suggest involvement in cellular processes but the exact mechanical functions remain to be clearly elucidated.
Biological function summary
The protein FAM108A1 is potentially involved in cellular signaling and regulation without forming part of any complex well-defined so far in the literature. Researchers speculate this protein may play a part in the regulation of intracellular pathways that control cell growth and differentiation. Despite limited direct data its expression patterns suggest a biological significance in tissue-specific functions especially in neural connections.
Pathways
Researchers found the involvement of proteins similar to FAM108A1 in fundamental pathways like cell proliferation or intracellular communication. Though specific pathway involvement of FAM108A1 itself is still under investigation proteins like ERK1/2 often surface in studies as potentially interacting partners due to their roles in similar biological processes. Understanding its interaction with known pathways can bring clarity to FAM108A1's function.
Studies suggest an association between the expression patterns of FAM108A1 and certain neurological disorders and cancers. There could be connections to neurodegenerative disorders due to its higher expression in neurons although concrete disease mechanisms are not fully defined. Research into proteins like p53 which have established roles in cancer alongside FAM108A1 might reveal new insights into their mutual associations within these disorders.
