Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 7 bp deletion in exon 2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-COQ8B, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ADCK4 also known as aarF domain containing kinase 4 is a protein kinase with a molecular weight of approximately 49 kDa. It mainly exists in the mitochondria where it plays a significant role in cellular energy production. Expression of ADCK4 appears highest in the kidney and to a lesser extent in other tissues. As a kinase its mechanical function depends on phosphorylating various substrates which is necessary for the maintenance of mitochondrial function.
Biological function summary
ADCK4 participates in the biosynthesis of coenzyme Q10 a vital component of the electron transport chain. This protein forms part of a larger complex known as the coenzyme Q biosynthesis complex where it helps maintain cellular energy and metabolic balance. It interacts closely with other proteins involved in this pathway to regulate coenzyme Q10 levels which affects multiple cellular processes including ATP production.
Pathways
ADCK4 significantly integrates into the mitochondrial respiratory chain pathway and the broader metabolic energy pathway. It aids in the synthesis of coenzyme Q10 alongside proteins like COQ6 and COQ7 directly impacting the efficiency of energy production in mitochondria. This involvement supports metabolic activities that are critical for cellular homeostasis and energy regulation.
Mutations or dysfunctions in ADCK4 correlate with nephrotic syndrome and coenzyme Q10 deficiency. Individuals with nephrotic syndrome often show altered ADCK4 activity which affects coenzyme Q10 synthesis and leads to kidney-related complications. Additionally connections between ADCK4 and the action of COQ2 indicate potential pathways through which mitochondrial and renal disorders might develop from inadequate coenzyme Q10 levels.
