Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon4.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ADCY7, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ADCY7 also known as Adenylate Cyclase 7 is an enzyme responsible for the conversion of ATP to cyclic AMP (cAMP) an important secondary messenger in cells. This enzyme has a molecular mass of approximately 120 kDa. ADCY7 is expressed mainly in the brain and immune cells such as T cells and B cells. In cellular membranes ADCY7 functions as part of a larger signaling network contributing to the regulation of various cellular processes.
Biological function summary
This enzyme participates in the modulation of intracellular signaling pathways by altering cAMP levels. ADCY7 does not usually function alone; it interacts with other signaling molecules forming complexes that influence cellular responses. By regulating cAMP levels ADCY7 can affect processes like cell proliferation differentiation and immune responses.
Pathways
Adenylate cyclase activity of ADCY7 plays a significant role in G protein-coupled receptor (GPCR) signaling and is involved in the downstream cAMP signaling pathway. These pathways are critical for transmitting signals from extracellular stimuli to intracellular effectors. ADCY7 interacts with G proteins which directly influence its activity. It is also associated with pathways including the MAPK signaling involving a chain of proteins in the cell that communicates signals from receptors on the cell surface to the DNA in the nucleus.
Changes in ADCY7 function are linked to both immune and neurological conditions. For example aberrant ADCY7 signaling has been associated with mood disorders such as depression. Additionally abnormalities in its regulation could influence autoimmune disorders due to its expression in immune cells. ADCY7 connects to other proteins implicated in these conditions like G proteins which are involved in modifying immune and neurotransmitter receptor activities.
