Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon8 and 26 bp deletion in exon8.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-AKAP11, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AKAP 220 also known as AKAP 11 is a protein kinase A anchoring protein with a molecular mass of approximately 220 kDa. It plays an essential role in cellular signaling by tethering protein kinase A (PKA) to specific subcellular locations. You can find this protein expressed in many tissues but it is particularly abundant in the brain and testis. AKAP 220's distribution helps ensure that its regulatory functions occur in precise cellular regions enhancing the specificity of cAMP-mediated signaling.
Biological function summary
AKAP 220 anchors PKA and other signaling molecules to form a multi-protein complex. This complex facilitates the spatial and temporal regulation of PKA activity allowing cells to respond accurately to changes in cyclic AMP levels. AKAP 220 also interacts with other proteins such as phosphatases and cytoskeletal elements contributing to its role in modulating various cellular processes. It controls signal transduction pathways important for neuronal communication and reproduction due to its complex-forming ability.
Pathways
The protein plays an important role in the cAMP signaling and MAPK/ERK pathways. In these pathways AKAP 220 coordinates PKA with components like Ras and Raf essential players in cellular responses such as growth division and differentiation. By linking PKA to precise signaling cascades AKAP 220 influences the modulation of cellular processes that these pathways regulate impacting how cells react to external signals.
AKAP 220 shows a connection to neurodegenerative diseases and fertility issues. Altered expression or function of AKAP 220 has implications in disorders like Alzheimer's disease where its interaction with PKA and other signaling molecules might disrupt critical neuronal pathways. Additionally links exist between AKAP 220 and proteins like CREB which influences processes involved in memory and cognition further pointing towards its role in neurodegenerative conditions. Meanwhile in the context of fertility its abundance in testicular tissue suggests it impacts sperm motility and other reproductive functions.
