Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon3 and 5 bp deletion in exon3.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ANGPTL6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ANGPTL6 also known as Angiopoietin-like 6 is a protein involved in modulating several physiological processes. The protein has a molecular weight of approximately 54 kDa. ANGPTL6 is expressed in various tissues such as the liver heart and adipose tissue indicating its involvement in systemic functions. The protein is a secretory glycoprotein influencing various cellular mechanisms due to its diverse localization.
Biological function summary
ANGPTL6 functions in the regulation of metabolic processes including glucose and lipid metabolism. It is not part of a larger complex but acts individually in its biological roles. ANGPTL6 has been shown to improve insulin sensitivity and promote energy expenditure. Through these mechanisms it plays an essential role in maintaining metabolic homeostasis and supports cellular health.
Pathways
ANGPTL6 significantly interacts within metabolic and cardiovascular pathways. The protein influences the insulin signaling pathway and is connected to proteins like AKT and PI3K integral components of metabolic regulation. ANGPTL6 also affects pathways involved in angiogenesis providing cross-talk with vascular endothelial growth factor (VEGF)-mediated signaling an important aspect of cardiovascular health.
ANGPTL6 has connections to metabolic syndrome and obesity-related conditions. Its role in maintaining insulin sensitivity and influencing glucose metabolism links it to type 2 diabetes. By modulating pathways related to lipid balance ANGPTL6 also relates to cardiovascular diseases. Associations with proteins such as VEGF and AKT elucidate its involvement in vascular health and metabolic disorders.
