Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 14 bp deletion in exon 4.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ANKRD52, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ANR52 also known as Ankyrin Repeat Domain-Containing Protein 52 is a protein characterized by a series of ankyrin repeat motifs. The protein has a molecular mass of approximately 75 kDa. ANR52 is expressed in various tissues with higher levels found in neuronal and immune cells. The protein plays a mechanical role in intracellular interactions facilitating protein-protein bindings through its ankyrin repeat domains. This enables it to act as a scaffold organizing specific receptor and signaling proteins.
Biological function summary
The function of ANR52 is to partake in cellular communication and signaling. ANR52 is involved as a part of a larger protein complex which aids in the localization and stabilization of proteins at the cell membrane. It interacts with key signaling molecules influencing pathways that regulate cellular processes such as proliferation and apoptosis. The role of ANR52 in maintaining cellular homeostasis is essential for proper cell function and health.
Pathways
The activities of ANR52 focus on regulation within the NF-kB and MAPK signaling pathways. These pathways oversee a variety of cellular responses including inflammation and stress responses. ANR52 interacts with proteins such as IKK and Raf forming connections that modulate the pathway's activity. By doing so ANR52 assists cells in adapting to environmental stimuli and maintaining physiological balance.
ANR52 shows connections to autoimmune diseases and neurodegenerative disorders. Its altered function or expression affects pathways involved in these conditions revealing links to diseases like rheumatoid arthritis and Alzheimer's disease. ANR52's relationship with other proteins such as TNF receptor-associated factors in rheumatoid arthritis highlights its role in pathogenic processes. These insights suggest potential therapeutic targets for modulating ANR52 activity to alleviate disease symptoms.
