Description
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 5 bp deletion in exon2.,
Disease:Carcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-APOL6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
APOL6 also known as apolipoprotein L6 is a protein with an approximate molecular mass of 41 kDa. Expressed primarily in the liver kidney and heart APOL6 plays a critical role in modulating lipid metabolism. As a member of the apolipoprotein L family APOL6 is involved in lipid binding and transport which are essential processes for maintaining lipid homeostasis in the cell.
Biological function summary
APOL6 influences important cellular processes such as apoptosis and lipid metabolic processes. Although not part of a large protein complex APOL6 contributes significantly to the regulation of phospholipid transport and stability within cellular membranes. Through these functions APOL6 supports cellular homeostasis and impacts the regulation of cell death pathways.
Pathways
Lipid metabolism and cell signaling heavily involve APOL6. It interacts with other apolipoproteins and factors like cholesterol transport proteins. Within the lipid metabolic pathway APOL6 facilitates proper lipid transport working closely with apolipoprotein E (ApoE) which is another key player in these processes. Disruption of this interaction can lead to imbalances in lipid homeostasis affecting overall health.
APOL6 has been linked to conditions like cardiovascular disease and specific forms of cancer. Its involvement in lipid metabolism and apoptosis suggests that alterations in APOL6 function or expression may lead to these health issues. APOL6 connects to proteins such as ApoE in cardiovascular diseases due to their shared roles in lipid transport and metabolism which directly affects plaque formation and cell health in blood vessels. Understanding these relationships helps in targeting therapeutic interventions.
