Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon7 and 2 bp deletion in exon7.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ARAP3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ARAP3 also known as ArfGAP with RhoGAP domain ankyrin repeat and PH domain 3 regulates actin cytoskeleton dynamics and cell signaling. This protein with a molecular mass of around 163 kDa functions as a GTPase-activating protein specifically for Arf6 and RhoA. ARAP3 is widely expressed in various tissues but shows higher expression levels in the brain and blood cells. It plays an essential role in cellular processes such as endocytosis and cell migration.
Biological function summary
ARAP3 modulates intracellular signaling pathways that control cytoskeletal rearrangement and cellular adhesion. It acts as part of a protein complex that includes PI3K and is influenced by phosphoinositide binding. This interaction helps facilitate growth factor-induced cell movement. Researchers observe that ARAP3 interacts with cell surface receptors to relay extracellular signals to cytoskeletal components affecting cellular dynamics.
Pathways
ARAP3 participates significantly in the PI3K/Akt pathway mediating membrane trafficking and signal transduction. Its activity impacts the regulation of Rho family GTPases central to controlling cell shape and motility. ARAP3 works together with Arf6 and plays a modulator role linking between actin remodeling and vesicle transport systems. This protein maintains the communication between PI3K signaling and actin reorganization driving cellular responses to external stimuli.
ARAP3 links to conditions affecting the immune system and cell growth. Abnormal ARAP3 activity associates with autoimmune diseases and certain cancers disrupting normal cell behavior. In these contexts ARAP3's interactions with proteins like Arf6 and elements in the PI3K signaling pathway become important. Its dysregulation may lead to uncontrolled cell proliferation or faulty immune responses positioning ARAP3 as a target for therapeutic strategies.
