Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ATP5F1E, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP5E also known as ATP synthase epsilon subunit is a component of the ATP synthase enzyme complex specifically the F1 portion. This protein has an approximate molecular mass of 5.6 kDa. ATP5E is mainly present in the inner mitochondrial membrane where it plays a role in ATP production. The synthesis of ATP is a process important for cellular energy and ATP5E contributes by stabilizing the structure of the ATP synthase complex.
Biological function summary
ATP5E functions as an integral part of the ATP synthase complex which comprises multiple subunits. This complex is essential for oxidative phosphorylation a primary method through which cells produce ATP. ATP5E's role is structural helping to keep the F1 catalytic core properly aligned during the conversion of ADP and inorganic phosphate into ATP. Through this biological activity ATP5E supports energy metabolism necessary for the survival of cells.
Pathways
ATP5E participates in the oxidative phosphorylation pathway a critical process for cellular respiration. This pathway involves electron transport and proton pumping processes that generate the proton gradient ATP synthase exploits to synthesize ATP. Other proteins like ATP5C1 the gamma subunit are closely related to ATP5E in this pathway each contributing to the efficient production of ATP from ADP and inorganic phosphate.
ATP5E has connections with mitochondrial disorders such as Leigh syndrome. This disease results from mutations affecting mitochondrial function leading to severe energy deficits in cells. Mutations in ATP5E can disrupt the ATP synthase complex’s function contributing to the pathology of these disorders. ATP5A1 another subunit of the ATP synthase complex also links with these diseases underlining the importance of all these components in maintaining mitochondrial integrity and function.
