Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ATP5MC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP5G2 also known as ATP synthase subunit c mitochondrial 2 plays a mechanical role in the production of ATP. This protein functions as a component of the Fo subunit of ATP synthase which operates in the mitochondrial membrane. ATP5G2 has a mass of approximately 8.2 kDa and appears in tissues with high energy demand like muscle and nerve cells. The protein's primary role is to facilitate proton translocation across the mitochondrial membrane important for ATP synthesis.
Biological function summary
ATP5G2 contributes to the generation of cellular energy by being part of the ATP synthase complex also called Complex V of the mitochondrial electron transport chain. This complex performs oxidative phosphorylation converting ADP and inorganic phosphate into ATP which fuels various cellular processes. The subunit's function within the complex directly impacts cellular energy yields and efficiency. Its interaction with other ATP synthase subunits is vital for maintaining the function of the entire complex.
Pathways
ATP5G2 integrates into the oxidative phosphorylation pathway which is central to energy metabolism. This pathway is important for the conversion of nutrients into usable energy. ATP5G2 works closely with the NADH dehydrogenase complex (Complex I) to enable effective electron transport and subsequent ATP generation. The intricate coordination between these complexes is necessary for the maintenance of the mitochondrial membrane potential and overall cellular energetics.
Mutations or dysfunction in ATP5G2 can link to mitochondrial disorders such as Leigh syndrome which involves neurodegeneration and energy deficits. The protein's malfunction can disrupt ATP synthesis affecting the energy balance in cells particularly those requiring high ATP levels. Additionally ATP5G2 might contribute to metabolic disorders like diabetes where insulin secretion and action depend on proper energy metabolism. The role of ATP5G2 alongside other ATP synthase subunits highlights its importance in maintaining mitochondrial and cellular function.
