Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2 and 31 bp deletion in exon2.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-DMAC2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ATP5SL also known as the ATP synthase subunit s-like protein interacts with components of the mitochondrial ATP synthase complex. This protein has a mass of approximately 27 kDa. Researchers often detect its expression in tissues with high metabolic rates such as the heart and skeletal muscles. ATP5SL participates in energy production by modulating the efficiency of ATP synthesis.
Biological function summary
The ATP5SL protein plays a significant role in ATP production acting within the mitochondrial ATP synthase complex. As part of this complex it helps synthesize ATP from ADP and inorganic phosphate. ATP5SL works closely with other subunits in the complex to maintain optimal energy conversion necessary for cellular functions. Its proper function is essential in maintaining cellular energy homeostasis.
Pathways
The role of ATP5SL is integral to oxidative phosphorylation and cellular respiration. It interacts with various proteins including ATP5A1 and ATP5B which are critical to the ATP synthase complex. These interactions ensure efficient electron transport chain function and ATP production key steps in cellular respiration and energy metabolism pathways.
ATP5SL can affect conditions related to mitochondrial dysfunctions like mitochondrial encephalomyopathy and cardiomyopathy. Abnormalities in ATP5SL can lead to impaired energy production. Disorders linked to the malfunctioning of ATP synthase often show associations with proteins like MFN2 which plays a role in mitochondrial fusion. Understanding ATP5SL's involvement in these processes provides insights into potential therapeutic targets for mitochondrial-related diseases.
