Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 11 bp deletion in exon2.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-ATRAID, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C2orf28 also called FLJ20492 is a protein encoded by a gene on chromosome 2 open reading frame 28. This protein has a molecular mass of approximately 20 kDa. It is chiefly expressed in various human tissues including the brain heart and liver. Mechanically C2orf28 interacts with other cellular components although its precise role in cellular processes is not fully defined yet.
Biological function summary
The C2orf28 protein contributes to intracellular signaling and regulatory processes. Although this protein is not widely recognized as part of a complex it participates in maintaining cellular homeostasis which involves a variety of regulatory functions. Due to its expression pattern and regulatory roles it is considered to have an impact at the cellular level.
Pathways
C2orf28 engages in several cell signaling and metabolic pathways. It notably influences pathways related to cellular stress responses and metabolic regulation. The protein may interact with other mediators like AKT1 and MTOR which are essential in these pathways. C2orf28's involvement in such pathways underlines its role in modulating cellular dynamics.
C2orf28 is linked with neurodegenerative conditions and metabolic disorders. Research indicates its potential involvement in Alzheimer's disease due to its expression in the brain and interaction with proteins like tau. Additionally its role in metabolic regulation suggests a connection to disorders such as diabetes where it may interact with proteins involved in insulin signaling. Further studies could elucidate the precise mechanisms of these associations.
