Description
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.,
Disease:Adenocarcinoma
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-BOD1, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Bod1 also known as Biorientation Defective 1 is a protein with a mass of approximately 34 kDa. It is expressed in many tissues with notable presence in proliferating cells. Bod1 localizes mainly to the centrosomes and kinetochores during cell division. As a protein with important mitotic functions it contributes key mechanical roles at the cellular level ensuring proper chromosomal alignment and segregation.
Biological function summary
In regulating the mitotic spindle checkpoint Bod1 plays a significant role in securing accurate chromosome segregation. It forms part of the vital chromosomal passenger complex which includes proteins like Aurora B kinase survivin and borealin. The complex is essential for correcting improper microtubule-kinetochore attachments and achieving chromosome bi-orientation during cell division. Bod1 also assists in maintaining the structure and function of centromeres ensuring genetic stability.
Pathways
Bod1 interacts in the important processes of mitosis and spindle assembly checkpoint. It ensures correct chromosome alignment by acting in coordination with proteins such as PLK1 and Aurora B kinase which are essential for the maintenance of genomic integrity. The pathways that involve Bod1 are critical in regulating cell cycle transitions and protecting against aneuploidy a condition often arising from improper chromosome segregation.
Bod1 has been associated with certain cancers given its role in maintaining chromosomal stability. The protein's malfunction may promote aneuploidy leading to tumorigenesis. Bod1's interaction with Aurora B kinase links it to colorectal cancer where disruptions in mitotic processes play a role. Research suggests that targeting Bod1 could offer therapeutic strategies against cancers involving these mitotic dysregulations.
