Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon1.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-FAM174C, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C19orf24 also known as C19orf71 or Chromosome 19 Open Reading Frame 24 is a protein that participates in key cellular mechanisms. This protein weighs approximately 13 kDa. It is primarily expressed in several tissues including the liver lung and spleen. Its exact mechanical role remains under investigation but current research indicates that it plays important roles in cellular signaling and regulatory functions.
Biological function summary
The protein encoded by C19orf24 has interactions that suggest roles in cellular and molecular processes. It is not part of a known larger protein complex. Evidence suggests involvement in cytokine signaling and response making it an interesting target for studies focused on immune response and inflammation. The identification of specific binding partners remains an ongoing area of research as understanding these interactions could clarify its biological roles further.
Pathways
Multiple cytokine mediated signaling pathways involve C19orf24 especially in relation to immune system regulation. It contributes to key pathways like the IL-6 signaling which is important for inflammatory response and possibly interacts with related proteins such as STAT3 a well-known signaling molecule in immune reactions. Continued mapping of these pathways will help elucidate the broader impact of C19orf24 on cellular functions.
Current studies connect C19orf24 to autoimmune conditions and inflammatory disorders such as rheumatoid arthritis. The protein's potential interaction with cytokine pathways and proteins like TNF-alpha which play significant roles in inflammatory responses hints at its contribution to these diseases. Further examination is required to fully understand C19orf24's impact on disease progression and its potential as a therapeutic target.
