Description
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 5 bp deletion in exon4 and Insertion of the selection cassette in exon4.
Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages
Properties and Storage Information:
Gene name-C19orf48, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C
Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
C19orf48 also known as Chromosome 19 Open Reading Frame 48 is a protein encoded by the C19orf48 gene. This protein has a molecular mass of approximately 17.5 kDa. It has been found to be expressed in various tissues with notable levels in the brain and immune tissues. The precise mechanism of its action remains under investigation but it has been suggested to play a role in cellular processes related to transcription regulation.
Biological function summary
C19orf48 functions within the context of transcriptional control. While it is not currently identified as part of a large protein complex emerging evidence hints at its involvement in modulating the expression of certain genes. This implies a potential influence on cellular decision-making processes. By impacting gene expression C19orf48 may contribute to a variety of cellular outcomes many of which are relevant to the function of the nervous and immune systems.
Pathways
Studies indicate that C19orf48 participates in pathways associated with gene expression regulation and immune response. It is thought to have a connection with the NF-κB signaling pathway a critical mediator of immune and inflammatory responses. The protein may act alongside others like NFKBIA which is known to inhibit this pathway under specific conditions. Understanding of these interactions helps elucidate the broader roles that C19orf48 may play in cellular signaling networks.
Research has associated C19orf48 with neurological and immune-related conditions. Altered expression levels have been observed in certain types of cancers especially in gliomas suggesting a potential role in tumorigenesis. Additionally connections with proteins such as p53 have been noted which are important in cancer biology due to their involvement in cell cycle regulation and apoptosis. Further investigation is necessary to fully characterize these relationships and their implications for disease progression and therapy.
